This book provides a concise introduction to the qualitative and quantitative analysis of substances of biochemical importance for undergraduate medical students. It covers all the skill-based aspects of the CBME curriculum for biochemistry.
Rafi MD, Dean and Medical Director of Surabhi Institute of Medical Sciences, Siddipet, obtained his MBBS degree from Kakatiya Medical College, Warangal, and MD from Gandhi Medical College, Hyderabad. He is known for his passion for maximising the learning experience for his students through innovative teaching methods. Dr Rafi has several research publications to his credit in journals of repute.
1. Safety in the Laboratory Precautions First Aid in the Laboratory
QUALITATIVE EXPERIMENTS 2. Reactions of Non-protein Nitrogenous (NPN) Substances Urea Uric Acid Creatinine Identification of unknown NPN substances 3. Analysis of Urine Physical Examination Microscopic Examination Chemical Analysis 4. Analysis of Abnormal Constituents of Urine Glucose Ketone Bodies Proteins Blood Bile Salts Bile Pigments Identification of unknown abnormal constituents in urine
QUANTITATIVE EXPERIMENTS 5. Clinical Biochemistry Laboratory: Instrumentation and Techniques Glassware and Instruments Commonly Used in the Laboratory Solutions Sample Collection and Handling Colorimetry Spectrophotometer Autoanalyser Flame Photometer Fluorimeter Dry Chemistry Systems ABG Analysis Immunodiffusion Quality Control 6. Blood Glucose Sample Collection Determination of Glucose in Body Fluids 7. Glucose Tolerance Test Indications for GTT Glucose Tolerance Curve 8. Blood Urea and BUN Methods of Blood Urea Estimation Urinary Urea and Urea Clearance 9. Serum and Urinary Creatinine and Creatinine Clearance Creatine and Creatinine Creatinine Clearance 10. Uric Acid Formation and Excretion of Uric Acid Methods of Estimation 11. Serum Total Proteins and Albumin–Globulin Ratio 12. Cholesterol and Lipid Profile 13. Bilirubin Serum Bilirubin 14. Aminotransferases (AST and ALT or SGOT and SGPT) 15. Alkaline and Acid Phosphatases (ALP and ACP) Estimation of Alkaline Phosphatase Estimation of Acid Phosphatase 16. Creatine Kinase and Its Isoenzymes 17. Amylase and Lipase Estimation of Serum Amylase Estimation of Serum Lipase 18. Calcium and Phosphorus 19. Serum Electrolytes Electrolyte Analysis by Ion Selective Electrode 20. Quantitative Analysis of CSF 21. Electrophoresis Types of Electrophoresis 22. Chromatography 23. Radioimmunoassay (RIA) and Enzyme-linked Immunosorbant Assay RadioImmunoassay (RIA) Enzyme-linked Immunosorbant Assay (ELISA) 24. Estimation of pH and Preparation of Buffers Determination of pH Using a pH Meter Buffers 25. Biomedical Waste Management Segregation of Waste Storage of Waste Transportation of Waste Treatment and Disposal of Waste Safety Measures 26. DNA Isolation and Quantification Basic Steps of DNA Isolation Quantification of DNA 27. Biochemical Tests for Common Clinical Conditions Diabetes Mellitus Dyslipidemia Myocardial Infarction Renal Failure Gout Proteinuria Nephrotic Syndrome Edema Jaundice Liver Disorders Pancreatitis Acid-Base Disorders Thyroid Disorders Vitamins and Minerals 28. Urinary Screening for Inborn Errors of Metabolism 29. Energy Content of Various Foods and the Significance of Glycemic Index Energy Requirements in Humans Energy Content of Foods Glycemic Index 30. Saturated, Unsaturated and Trans-Fatty Acids in Foods Saturated Fatty Acids Unsaturated Fatty Acids Biomedical Importance of PUFA Trans-fats
APPENDICES I. Practical Biochemistry Question Bank Qualitative Experiments Quantitative Experiments II. Multiple-choice Questions (MCQs) in Practical Biochemistry III. Problem-solving Exercises, Interpretation of Laboratory Data and Case Studies in Practical Biochemistry IV. Test Procedures of Qualitative and Quantitative Experiments in a Nutshell Qualitative Test Procedures (in alphabetical order) Quantitative Test Procedures Spotters and Charts